Root-knot nematodes (Meloidogyne spp.) are among the most economically important plant-parasitic nematodes, causing severe yield losses in a wide range of crops worldwide . Excessive reliance on chemical nematicides has led to environmental concerns and regulatory restrictions, necessitating the development of eco-friendly alternatives. Xenorhabdus spp., symbiotically associated with entomopathogenic nematodes, produce a diverse array of bioactive metabolites collectively referred to as Xenotox, which exhibit nematicidal, insecticidal, and antimicrobial properties. This article reviews the potential of Xenorhabdus toxin (Xenotox) for the management of root-knot nematodes in different crops, emphasizing its efficacy, mode of action, and role in sustainable nematode management.