Effective disease control and sustaining agricultural productivity depend heavily on the prompt and accurate detection of plant viral infections. Our capacity to identify and track viral infections in agricultural crops has greatly increased because of recent developments in the detection of plant viruses. In order to minimize crop losses and stop viral infections from spreading to healthy plants, quick and accurate detection techniques are crucial. Additionally, the development of virus-free planting material, safe foreign travel and the screening of germplasm at quarantine facilities all depend heavily on diagnostic technologies. Due to its great sensitivity and accuracy, ELISA is one of the most important protein-based diagnostic techniques for plant pathogen identification. When many samples need to be examined simultaneously, it is extremely helpful. Thus, stakeholders would be able to select the best management methods against viral risks and guarantee global food security if they were aware of the key characteristics of the diagnostic methodologies now in use.