Probit analysis is commonly used in concentration dose response bioassays to study mortality or survival. Bioassay data usually show a curved relationship between dose and response, which is difficult to analyze directly. Probit analysis simplifies this by converting response percentages into probit values and relating them to log-transformed doses, resulting in an almost straight-line relationship. This helps in estimating concentrations or doses that produce specific levels of biological effect with good accuracy. The method also provides information on the rate of increase in mortality, variability in responses and how well the data fit the model. These outputs allow reliable comparison of toxicity among different treatments and populations. Because of its simplicity and reliability, probit analysis remains a standard method for analyzing dose?mortality relationships in toxicological and entomological studies.