CRISPR/Cas9 has emerged as a breakthrough site-specific genome-editing technique due to its ease of use, simplicity, and high efficiency that has revolutionized the field of genome editing. It allows scientists to create transgene-free genome-edited plants by allowing them to target and modify specific DNA sequences. The foremost step designing this system in plants is to identify the target gene followed by designing the construct which includes identification of appropriate Cas proteins, design and selection gRNAs, and selection of regulatory elements to express gRNAs and Cas proteins. It complexes with sgRNA for DNA targeting and requires PAM site downstream of its target sequence for DNA recognition. Once Cas9 recognizes its PAM sequence, the Cas9-sgRNA complex binds to the target sequence and generates a DSB at the target site. DNA cleavage activity of Cas9 is achieved by the combined effort of two parts of the protein (RuvC and HNH). In most genome editing experiments, the gRNA as well as the Cas9 and selectable marker genes, have been delivered into plant cells using either T-DNA (Agrobacterium infection) or plasmid DNA (particle bombardment). For most purposes, validation and characterization of edits on both the molecular and phenotypic level, will be required to assess their biological relevance. The CRISPR/Cas9 system has been successfully applied in various plant species. These include not only model plants, such as Arabidopsis, but also crops, such as rice, tobacco, sorghum, wheat, maize, soybean, tomato, potato, poplar, apple and banana.