Seed viability is the ability of seed to germinate which is evidenced by a number of physiological or biochemical events. Paper media can be used for assessing seed viability. This study intended to discover the most effective technique of seed germination utilizing paper substrate for the viability of sesame seeds. The research was conducted at the Seed Laboratory of Directorate of rapeseed and mustard research centre, Bharatpur (Raj). The study approach used a two-factor factorial Completely Randomized Design. The first component was the sesame seed variety which consisted of RT-346, RT-127, RT-125, RT-103, RT-54, RT-46, TKG-308. The second factor was the seed germination procedure which comprised of a test on top of paper, a test on filter paper. Each treatment was reproduced four times, generating 8 experimental units. Sesame seed viability was greatly affected by the way of seed germination utilizing paper media, according to the findings. In compared to other treatments, sesame seeds of the RT-46 variety displayed the best percentages of simultaneous growth, germination, vigor index, normal seedling dry weight, hypocotyl length, and radicle length when tested on filter paper.