Prolactin is synthesized from anterior pituitary and secreted highly during the diestrual phase of the estrous cycle in bitches. Various reproductive clinical conditions are arising during diestrual stage of bitches. Manipulation of the diestrual phase of the estrous cycle in a bitch, by a simple administration of anti-prolactin drugs could bring about changes in the diestrus period. Commonly used dopamine agonists in canine practice are cabergoline and bromocriptine. The present communication elaborates the role of prolactin and dopamine agonists in various clinical conditions of bitches.