In agriculture, biosensors are vital tools because they enable prompt and accurate identification of infections, contaminants, and fraudulent food. They are composed of a biological sensing element and a transducer for the detection of analyte concentration. Many biosensors are available for biorecognition components, including aptamers, enzymes, nucleic acids, and antibodies. Among the many uses of biosensors in agriculture are the direct identification of plant pathogens, early disease detection, soil chemistry, air organophosphorus concentrations, pesticides, plant nitrate status, and foodborne pathogens. Biosensors can be an important addition to the components which helps in achieving sustainable agriculture practices.