The utilization of fish-based collagen is growing fast due to its unique properties in comparison with mammalian-based collagen. Fish based collagen has no risk of transmitting diseases, a lack of religious constraints, a cost-effective process, low molecular weight, biocompatibility, and its easy absorption by the human body. Collagen is the most abundant structural protein in the extracellular matrix of the various connective tissues in the body such as skin, bones, ligaments, tendons, and cartilage. The application of collagen is used as biomaterials, especially as drug and gene carriers, tissue engineering, absorbable surgical suture, osteogenic and bone filling materials, hemostatic agents, immobilization of therapeutic enzymes etc.