Mulberry (Morus spp.) is a critical plant for sericulture, serving as the exclusive feed for the silkworm (Bombyx mori L.). The propagation of high-quality, genetically uniform mulberry plants is essential to meet the growing demand for sericulture. Mini clonal technology has become a reliable method for large-scale propagation of mulberry thereby offering efficient multiplication of elite clones. However, the success of mini clonal technology is highly dependent on environmental factors particularly relative humidity (RH). RH plays a vital role in various physiological processes including water retention, transpiration and root development which will directly influence the survival of mulberry mini clones during propagation. This article explores the complex interactions between RH and plant physiology and their collective impact on the survivability of mulberry mini clones propagated through mini clonal technology.